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KMID : 1145720140020020033
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Korean Herbal Medicine Informatics
2014 Volume.2 No. 2 p.33 ~ p.39
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Authentication of the Constituents from Unknown Herbal Pill Using DNA Barcode Analysis and GenBank Database
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Moon Byeong-Cheol
Seo Bo-Young
Oh Hye-Hyun
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Abstract
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The herbal pill is usually made into several kinds of herbal medicines and supplementary excipients, such as starch, wheat flour, and honey. However, the verification of correct constituents of herbal pill is very difficult because it is composed of powders mixed with diverse herbal medicines. To determine the material composition of an unknown herbal pill, we analyzed DNA barcode sequences and NCBI GenBank nucleotide database on trial. Among the several DNA barcodes, nuclear ribosomal RNA gene internal transcribed spacer 2 region (ITS2) amplified sufficient PCR products to analyze the nucleotide sequences and provided valuable informations to determine the constituents of herbal pill on GenBank blast search. These valuable information is enough to estimate approximately the main herbal medicines as well as excipients. Therefore, we suggest DNA barcode sequence analysis depending on the GenBank database as a tool of quality control method with chemical analysis for herbal pills.
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KEYWORD
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DNA barcode, Herbal pill, GenBank database, Internal transcribed spacer 2 (ITS2)
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